CoAlab/角质形成细胞转谷氨酰胺酶（TG1）（hu）/150 ug/pro0001
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| Product Code | pro0001 |
|---|---|
| Uniprot ID | P22735 |
| Name | Transglutaminase-1 (TGase-1) |
| Appearance | White lyophilized solid |
|---|---|
| Constituents | The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion. |
| Conc. / Activity | 8201 U/mg |
| Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawingDelivery at ambient temperature is possible |
| Description | His6-rhTG1 is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. His6-rhTG1 is purified by IMAC to more than 90 % purity. |
| Produced in | Insect cells |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7051 |
| Labelling | His-Tag |
| Preparation method | Add 10 mM Ca2+ to activate His6-rhTG1. |
| Molecular weight | 90 kDa |
| Principle | His6-rhTG1 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG1 may also be used for immunoprecipitation. |
| Purity | > 90 % (visually by SDS-PAGE) |
| Recommendations | Add 340 µl of distilled water to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage. Protein concentration (determined on reconstituted rhTG1): 0.46 mg/ml |
| Product type | Proteins |
| Applications | Immunoprecipitation, Functional Assay |
品牌介绍
covalab肽合成 凭借我们在肽设计，合成和修饰领域的专业知识，我们提供了高质量的肽合成服务，包括广泛的化学修饰。我们的肽是使用固相Fmoc化学和全自动多肽合成仪合成的。我们提供具有大范围纯度级别，数量和N端，C端或侧链化学修饰的合成肽。每种肽都提供了反相HPLC和质谱数据，以验证肽的组成和纯度。
