covalab/Covalight ECL试剂/1 x(250 ml+15 ml)/opr0009
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产品分类:
逆转录试剂盒
公司分类:
Sensiscript_RT_Kit
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3392242852
电话号码:
4000-520-616
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info@ebiomall.com
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Product Code | opr0009 |
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Constituents | Reagent Cov A Reagent Cov B |
Lysat Type | "0 |
Principle | Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxidase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on spontaneous change to a stable form emits light. A dramatic increase in the light output can be achieved by the presence of enhancers. |
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Applications | Western Blot, Dot Blot |
Recommendations | Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxydase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on s |
Storage | Stored at +4°C |
Product type | Reagents |
Preparation method | To be used extemporaneously or can be kept at +4°C for few hours : Add 1 drop of oxidant Cov B to 1 mL of substrat Cov A (125 µL of reagent covers 1 cm2 membrane). Detection : Immerse the membrane in Covalight reagent from 1 to 10 minutes. Shake of excess reagent and cover the membrane with a plastic sheet before to exposure. |
* Lassot I et al. Cell Rep. 2018 Nov 27:25(9):2484-2496.e9* LM Bloyet et al.Sci. Adv. 2019, 5 eaaw3702* Lassot I et al. Cell Rep. 2018 Nov 27:25(9):2484-2496* Chapuy-Regaud S et al. Biochimie. 2017 May 5.* Brosse A et al. Nucl. Acids Res. 2016 Jul 20.* Bloyet LM et al. J Virol. 2016 May 11.* Fischer K. TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Ph.D. Thesis 2015.* Julien M et al. Biochim Biophys Acta. 2015 Jun 14. pii: S0167-4889(15)00193-7.* Romanets-Korbut O et al. Cell Mol Immunol. 2015 Jun 15.* Couderc C. et al. Mol Cell Endocrinol. 2014 Sep 16. pii:S0303-7207(14)00284-6.* Schnorr S et al. Pain. 2014 Jun:155(6):1079-90.* Brot S, et al. Eur J Neurosci. 2014 Jul 12.* Yakoub S et al. J Cancer Sci Ther 2014, 6.4* Bollard J et al. Neuroendocrinology. 2013 Jan 23.* Perez Alea M et al. Amino Acids. 2013 Jan:44(1):245-50* Chauvelier-Grouiller S. Caractérisation épigénétique du lymphome du manteau. Ph.D. Thesis 2011
- Three differents ECL Reagent were used with an exposure times of 2 min 30.1: Covalight® (opr0009)2: Covalight® Plus (opr1102) 3: Covalight® Xtra (opr1101)">
品牌介绍
covalab肽合成 凭借我们在肽设计,合成和修饰领域的专业知识,我们提供了高质量的肽合成服务,包括广泛的化学修饰。我们的肽是使用固相Fmoc化学和全自动多肽合成仪合成的。我们提供具有大范围纯度级别,数量和N端,C端或侧链化学修饰的合成肽。每种肽都提供了反相HPLC和质谱数据,以验证肽的组成和纯度。